WebBrain endothelial cells (BECs) are important conduits that deliver oxygen and nutrients, protect parenchyma cells from toxins, and drain wastes to maintain brain homeostasis. Impairment of BECs has been implicated in diverse neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. Therefore, molecular analysis of BECs is … WebGrowth-associated protein 43 (GAP43) is one compound used to indicate growth of axonal endings during development and regeneration, particularly of peripheral neurons. Using immunohistochemistry, we have studied the expression of GAP43 in the spinal cord of rats subjected to mild, moderate or severe …
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WebOverall design All DRG neurons from young adult (3-6 mo) male mice expressing Rpl22HA/HA Runx3-Cre+ and age/sex matched Cre negative littermates (Rpl22HA/HA Runx3-Cre-) were harvested using density purification on Percoll and grown for 24h in serum-supplemented F12 medium. WebRNA from whole striatal tissue was extracted for IP of Rpl22HA: sequencing of IP and input material was performed (“RiboTag AAV”). (D) Expression levels of cell-specific markers in RiboTag AAV IP samples (n = 4 mice). (E) Schematic of the protocol used. (F) Fold-change of pan reactive, A1 and A2 astrocyte specific markers between hPMCA2w/b ... links contracting
Stress gates an astrocytic energy reservoir to impair …
WebDec 21, 2016 · Finally, we crossed the Aldh1l1-Cre/ERT2 mice with RiboTag mice that express the ribosomal protein Rpl22HA in a Cre-dependent manner (Sanz et al., 2009). We used this strategy to determine the cortical astrocyte transcriptome in adult mice, which we compared to data from postnatal day 7 (P7) mice (Zhang et al., 2014 ). WebJan 7, 2024 · Crossing RiboTag mice (RPL22 HA/WT) with specific Cre-driver mice: Cre-lox recombination approach is commonly used as a tool for cell-type-specific gene expression. The RiboTag mice express HA-tagged ribosome protein RPL22 in Cre-expressing cells. WebJun 1, 2024 · The Rpl22-HA gene can also be introduced into the animal by stereotaxic injection of an AAV-DIO-Rpl22-HA that is then activated in Cre-expressing cells. Both … hourly fee only financial planner