WebbpHIS1525巨大芽孢杆菌表达质粒使用说明: 1、收到质粒干粉后请先5000rpm离心1min,再加入20μl无菌水溶解质粒,室温放置1min; 2、从-80℃冰箱中取出相应的感 … WebbThe aim of this research was to express the β-xylosidase gene in the pHIS1525/ Bacillus megaterium MS941 system. The xyl genewas amplified from pTP510 and... DOAJ is a unique and extensive index of diverse open access journals from around the world, driven by a growing community, committed to ensuring quality content is freely available online …
求助PHis1525质粒可交换 - 分子生物 - 综合其他 - 小木虫论坛-学术 …
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木糖诱导启动子及其质粒载体和应用的制作方法
http://www.bioon.com.cn/doc/showarticle.asp?newsid=96925 http://www.biofeng.com/zaiti/qita/pHIS1525.html WebbFor the construction of pHIS1525, fragments from XhoI and BglII digested pMM1525 and pHIS1522 were combined and ligated. The affinity tags of pHIS1525 and pSTREP1525 were combined in one vector using the outlined strategy resulting in pSTREPHIS1525. Next the part of the L. reuteri levansucrase gene, encoding an active and soluble protein in E. small to from stamp