Chlamydomonas hap2
Web1 Introduction. Chlamydomonas is an excellent model system to study the regulation of cilia and flagella. All major structural components of cilia are conserved in this unicellular … WebHAP2 is a class II gamete fusogen in many eukaryotic kingdoms. A crystal structure of Chlamydomonas HAP2 shows a trimeric fusion state. Domains D1, D2.1 and D2.2 line the 3-fold axis; D3 and a ...
Chlamydomonas hap2
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Webtransformed Chlamydomonas wild-type (WT) or mutant HAP2 transgenes carrying an influenza virus hemagglutinin tag (HA) into a fusion-defective, hap2 mutant strain (Liu et al., 2008) and assessed HAP2-HA expression and trafficking to the mating structure as well as fusion of the transformed hap2 minus gametes with WT plus gametes. HAP2-HA was ... WebMar 1, 2015 · Early reports on HAP2 in Arabidopsis concluded that it was required for gamete attachment (Mori et al., 2006), but by exploiting the ease of experimentally identifying and quantifying the location and properties of HAP2 at individual steps in gamete fusion in the green alga Chlamydomonas and the malaria pathogen Plasmodium …
WebFeb 23, 2024 · Studies in Chlamydomonas and Plasmodium (the pathogen causing malaria in humans) revealed that hap2 mutant gametes in both organisms were fully capable of … http://prodata.swmed.edu/Lab/HAP2.pdf
WebOct 3, 2024 · HAP2 is a class II gamete fusogen in many eukaryotic kingdoms. A crystal structure of Chlamydomonas HAP2 shows a trimeric fusion state. Domains D1, D2.1 and D2.2 line the 3-fold axis; D3 and a stem pack against the outer surface. Surprisingly, hydrogen-deuterium exchange shows that surfaces of D1, D2.2 and D3 closest to the 3 … http://genesdev.cshlp.org/content/22/8/1051.full
WebNov 22, 2024 · Gamete fusion in organisms across kingdoms depends on fusogen HAP2. Pinello et al. demonstrate that receptor MAR1 is associated with HAP2 on Chlamydomonas minus gametes and binds to conserved adhesion protein FUS1/GEX2 on plus gametes, suggesting a mechanism that ensures that bilayer merger is triggered only …
WebMar 23, 2010 · HAP2/GCS1 family members mediate membrane fusion between gametes in a broad range of eukaryotes, ranging from algae and higher plants to protozoans and … in christ latin translationWebJan 1, 2024 · Hydroxyproline-rich GP1 as an anchor for Chlamydomonas cell wall ... The screening experiment with pJPZ11_HAP2 did not result in any positive candidate, the outcome was confirmed by 50 mL cultures of the top candidate (Supp. Fig. S.3). The maximum RFU obtained during the screening in the 96 well plates for pJPZ11_HAP2 … incarnate the movieWebThe structure of Chlamydomonas HAP2 suggests that the cd loop is bipartite, with the two parts split by a small alpha helix (Fe´dry et al., 2024). Given this specific structure/function biological activity observed during the study of HAP2 in Tetrahymena, Arabidopsis, and Chlamydomonas, and the general conservation of the protein incarnate threads city of heroesWebChlamydomonas HAP2 is a member of a broadly conserved protein family whose founding member was identified in Arabidopsis in a screen for male sterile mutants (Johnson et al., 2004). [HAP2 more recently has also been termed GCS1 (Mori et al., 2006).] Previously, using gene disruption methods, we showed that in both Chlamydomonas and the rodent in christ in spanishWebFeb 23, 2024 · "The HAP2 protein from Chlamydomonas is folded in an identical fashion to the viral proteins," Rey said, referring to the molecular folding that creates the three-dimensional structure of all ... incarnate trilogyWebOct 3, 2024 · HAP2 is a class II gamete fusogen in many eukaryotic kingdoms. A crystal structure of Chlamydomonas HAP2 shows a trimeric fusion state. Domains D1, D2.1 and D2.2 line the 3-fold axis; D3 and a ... incarnate thundurusWebMay 3, 2024 · HAP2 is a class II gamete fusogen in many eukaryotic kingdoms. A crystal structure of Chlamydomonas HAP2 shows a trimeric fusion state. Domains D1, D2.1 … incarnate vs hou chri